Adhesive Compositions containing Dual Function Stabilizers and Active Agents

Adhesive Compositions containing Dual Function Stabilizers and Active Agents

US20030082116
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An adhesive composition includes a polymerizable adhesive monomer and at least one dual finction stabilizer. The stabilizer provides superior stabilization and shelf-life of the composition, and enhances wound healing properties of the monomer composition.

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(19) United States US 20030082116A1 (12) Patent Application Publication (10) Pub. No.: US 2003/0082116 A1 Badejo et al. (43) Pub. Date: May 1, 2003 (54) ADHESIVE COMPOSITIONS CONTAINING DUAL FUNCTION STABILIZERS AND ACTIVE AGENTS (75) Inventors: Ibraheem Badejo, Morrisville, NC (US); Anthony Sherbondy, Raleigh, NC (US); Timothy McAfoos, Wooster, OH (US) Correspondence Address: OLIFF & BERRIDGE, PLC P.O. BOX 19928 ALEXANDRIA, VA 22320 (US) (73) Assignee: CLOSURE MEDICAL CORPORA- TION, Raleigh, NC (US) (21) Appl. No.: 09/964,415 (22) Filed: Sep. 28, 2001 Publication Classification (51) Int. Cl.7 ........................... ..A61K 7/26; A61K 35/78 (52) U.S. Cl. .......................... .. 424/58; 523/120; 424/728; 424/730; 424/764; 424/765; 424/745; 424/748; 424/770; 424/736; 424/757; 424/754 (57) ABSTRACT An adhesive composition includes a polymerizable adhesive monomer and at least one dual function stabilizer. The stabilizer provides superior stabilization and shelf-life of the composition, and enhances Wound healing properties of the monomer composition. US 2003/0082116 A1 ADHESIVE COMPOSITIONS CONTAINING DUAL FUNCTION STABILIZERS AND ACTIVE AGENTS BACKGROUND OF THE INVENTION [0001] 1. Field of Invention [0002] The present invention relates to monomer and polymer adhesive and sealant compositions, and to their production and use for industrial and medical applications. In particular, the present invention relates to the incorpora- tion of dual function agents in adhesive compositions, such as to provide combined stabilization properties to the com- position and active agent properties to the application sur- face such as to enhance the wound healing properties of such compositions when used for medical purposes. [0003] 2. Description of Related Art [0004] Monomer and polymer adhesives are used in both industrial (including household) and medical applications. Included among these adhesives are the 1,1-disubstituted ethylene monomers and polymers, such as the ot-cyanoacry- lates. Since the discovery of the adhesive properties of such monomers and polymers, they have found wide use due to the speed with which they cure, the strength of the resulting bond formed, and their relative ease of use. These charac- teristics have made the ot-cyanoacrylate adhesives the pri- mary choice for numerous applications such as bonding plastics, rubbers, glass, metals, wood, and, more recently, biological tissues. [0005] It is known that monomeric forms of ot-cyanoacry- lates are extremely reactive, polymerizing rapidly in the presence of even minute amounts of an initiator, including moisture present in the air or on moist surfaces such as animal (including human) tissue. Monomers of ot-cy- anoacrylates are anionically polymerizable or free radical polymerizable, or polymerizable by zwitterions or ion pairs to form polymers. Once polymerization has been initiated, the cure rate can be very rapid. [0006] Medical applications of 1,1-disubstituted ethylene adhesive compositions include use as an alternate or an adjunct to surgical sutures and/or staples in wound closure, as well as for covering and protecting surface wounds such as lacerations, abrasions, burns, stomatitis, sores, minor cuts and scrapes, and other wounds. When an adhesive is applied to surfaces to be joined, it is usually applied in its mono- meric form, and the resultant polymerization gives rise to the desired adhesive bond. [0007] The industrial production of 1,1-disubstituted eth- ylene monomer adhesive compositions has been optimized to provide adhesives with rapid cure rates and high bond strengths. However, the desire to provide an adhesive with a rapid cure rate has led to problems with shelf-life. The shelf-life of these adhesives is primarily related to stability (i.e., constancy of compositional nature), uncured physical properties, rate of cure of the adhesive, as well as final cured properties of the composition. For example, the shelf-life of a monomeric ot-cyanoacrylate composition is related to the amount of time the composition can be stored before unac- ceptable levels of polymerization occur. Unacceptable levels are indicated by a level of polymerization product that reduces the usefulness of the composition in the application for which it is produced. It is well known that monomeric forms of ot-cyanoacrylates polymerize rapidly in the pres- May 1, 2003 ence of even minute amounts of an initiator, and that once polymerization has been initiated, the rate of cure can be very rapid. Therefore, in order to obtain a monomeric ot-cyanoacrylate composition with an extended shelf-life, polymerization inhibitors such as anionic and free radical stabilizers are often added to the compositions. However, addition of such stabilizers can result in substantial retarda- tion of the cure rate of the composition. Therefore, in the production of industrial ot-cyanoacrylate adhesives, the amount of stabilizers added is minimized so that the cure rate is not adversely affected. [0008] Cyanoacrylate adhesives used in medical applica- tions preferably have a shelf-life of at least twelve months. In order to achieve a useful shelf-life, anionic and free- radical stabilizers are generally added to the monomer compositions. [0009] As disclosed in U.S. Pat. Nos. 3,559,652 to Banitt et al. and 5,582,834 to Leung et al., for example, suitable stabilizers for medically useful ot-cyanoacrylate composi- tions include Lewis acids such as sulfur dioxide, nitric oxide, and boron trifluoride, as well as free-radical stabiliz- ers including hydroquinone, monomethyl ether hydro- quinone, nitrohydroquinone, catechol, and monoethyl ether hydroquinone. The combination of the two anionic stabiliz- ers sulfur dioxide and sulfonic acid is also known and is disclosed in, for example, British Patent Application GB 2 107 328 A. However, the use of these two anionic stabilizers in combination does not overcome the “speed loss” seen in other 1,1-disubstituted ethylene adhesive compositions. [0010] In addition to having an extended shelf-life, cyanoacrylate compositions for use in many medical appli- cations should be sterile. Due to the importance of achieving and maintaining sterility of these compositions, when an additive, such as an anionic or free-radical stabilizer, is added to an ot-cyanoacrylate composition, it should be added prior to sterilization. However, regardless of the type and number of additives, sterilization of ot-cyanoacrylate adhe- sive compositions is often difficult to achieve. For example, widely practiced methods of sterilization, such as heat sterilization and ionizing radiation, are often not suitable for use with monomeric cyanoacrylate compositions. Problems arise due to polymerization of the monomer during the sterilization process, even in the presence of stabilizers. In many cases, sterilization-induced polymerization is so severe that the resulting product is unusable. Furthermore, even when the sterilized product is still useable, the shelf- life at desired storage temperatures, such as under refriger- ated conditions or at room temperature, can be shortened to such a degree that the product is not suitable for commer- cialization. [0011] Methods currently used to package and sterilize ot-cyanoacrylate monomer compositions have been devel- oped with the recognition that, to improve efficiency and productivity, the packaging and sterilizing steps should be performed in rapid succession. However, these methods do not provide the desired shelf-life of the adhesive composi- tions in all packaging materials. [0012] Furthermore, during sterilization, much or all of the stabilizer can be consumed or converted to another com- pound. For example, U.S. Pat. No. 5,530,037 to McDonnell et al. discloses that when a low level of sulfur dioxide is used to stabilize a cyanoacrylate composition, all of the sulfur US 2003/0082116 A1 dioxide is converted to sulfuric acid during the sterilization process. Thus, although polymerization during sterilization can be minimized by use of low levels of sulfur dioxide, and shelf-life of he sterilized ot-cyanoacrylate adhesive compo- sition can be increased, shelf-life might be improved by the presence of increased amounts of sulfur dioxide in the initial composition. Unfortunately, at initial high levels of a stabi- lizer, the general performance of the adhesive can be impaired and the shelf life provided still is less than desired. [0013] McDonnell et al. also teaches that the use of the free radical stabilizers butylated hydroxyanisole (BHA) and butylated hydroxytoluene (BHT), in combination with 100 parts per million (final concentration) sulfur dioxide, are not effective at stabilizing ot-cyanoacrylate compositions during gamma irradiation sterilization. Rather, they must be present in concentrations substantially above 1000 parts per million or higher in order to provide effective stabilization. [0014] In addition, various phenolic stabilizers have been used for stabilizing adhesive compositions. For instance, McDonnell et al. teaches the use of a free radical stabilizer selected from phenolic antioxidants (except for hydro- quinone). U.S. Pat. No. 4,125,494 to Schoenberg teaches the use of anionic inhibitors including phenolic compounds such as hydroquinone, t-butyl catechol, pyrocatechol, p-methoxyphenol, and the like. U.S. Pat. No. 4,724,177 to Russo teaches the use of free radical stabilizers including hydroquinone, monomethylether hydroquinone, nitrohydro- quinone, and hydroquinone monoethylether. U.S. Pat. No. 5,034,456 to Katsumura teaches the use of radical polymer- ization inhibitors including hydroquinone, hydroquinone monomethyl ether, catechol, pyrogallol and the like. U.S. Pat. No. 4,321,180 to Kimura teaches the use of radical polymerization inhibitors such as aryl alcohols, phenol, cresols, hydroquinone, benzoquinone, ot-naphthol, [3-naph- thol, catechol, pyrogallol, bisphenol-A, bisphenol-S, 2,6-di- tert-butylphenol, 2,6-di-tert-butylcresol, 2,2'-methylene- bis(4-methyl-6-tert-butylphenol), 4,4'-butylidene-bis(3- methyl-6-tert-butylphenol), 4,4‘-thiobis(3-methyl-6-tert- butylphenol), hydroquinone monomethyl ether, 2-hydroxybenzophenone, phenylsalicylic acid, 1,3 ,5-trim- ethyl-2,4,6-tris(3,5-di-tert-butyl-4-hydroxybenzyl) benzene and the like. [0015] Thus, a need exists for improved monomer cyanoacrylate adhesive compositions, for both industrial and medical uses, having a longer shelf-life without sacrificing the performance of the adhesive. SUMMARY OF THE INVENTION [0016] The present invention provides an improved mono- meric adhesive composition, wherein the adhesive compo- sition comprise a selected dual-function stabilizer. In par- ticular embodiments, the present invention provides a monomer-containing adhesive composition comprising a polymerizable 1,1-disubstituted ethylene monomer, such as ot-cyanoacrylate monomer, and at least one selected dual function stabilizer. The dual function stabilizer provides, for example, stabilization effects to the composition, and wound healing effects when utilized for medical purposes. [0017] The combination of a polymerizable monomer with at least one selected dual function stabilizer according to the present invention provides an adhesive monomer composition with an enhanced and extended shelf-life and May 1, 2003 enhanced wound healing properties as compared to similar compositions lacking such additives. The present invention also provides an adhesive monomer composition with an enhanced and improved ability to withstand sterilization processing, such as gamma or electron beam irradiation processing, as compared to similar compositions lacking such additives. As used herein “extended shelf-life” refers to a shelf-life of at least 12 months, preferably at least 18 months, and even more preferably at least 30 months. Moreover, “enhanced wound healing capabilities” as used herein refers to the well known ability of various additives to increase the rate at which a wound heals, or to minimize the nature or effects of a wound. For example, where the dual function stabilizer is also an antioxidant, the antioxi- dant reduces free radicals that may otherwise hinder the ability of a wound to heal. Although it is known to add polymerization inhibitors (stabilizers) to monomeric adhe- sive compositions, the superiority of the use of a dual function stabilizer according to the present invention, to provide added stability and/or to enhance wound healing, has not been previously recognized. [0018] The present invention also includes a process for enhancing the wound healing properties of such adhesive compositions, and for increasing the shelf-life of such adhe- sive compositions. The enhancement of the wound healing properties of the compositions includes combining a poly- merizable monomer with a selected dual function stabilizer, either by itself or in combination with additional medica- ments or other additives. DETAILED DESCRIPTION OF EMBODIMENTS [0019] According to the present invention, a stable mono- meric adhesive composition is manufactured by combining at least one selected dual function stabilizer with a compo- sition comprising a monomer adhesive. The at least one selected dual function stabilizer according to the present invention 1) may inhibit polymerization of the monomer of the composition to a greater extent than can be achieved in prior art compositions, particularly during sterilization pro- cessing, and/or 2) may enhance the wound healing proper- ties of the adhesive when used in medical applications. [0020] The dual function stabilizer according to the present invention can be selected from among a range of materials that provide the desired stabilization and wound healing properties. For example, the dual function stabilizer can be selected from herbal extracts, alpha- and beta-C1-C30 hydroxycarboxylic acids, ceramides, anti-inflammatories, vasoconstrictors, and mixtures thereof. [0021] According to embodiments, the dual function sta- bilizer can be a selected herbal extract. Herbal extracts particularly suitable for the present invention are antioxi- dants or free-radical inhibitors. As used herein, an “antioxi- dant” means a compound that prevents degradation caused by oxidation. Preferably, the herbal extract is oil soluble rather than water soluble, although suitable results may be achieved using herbal extracts that are both oil- and water- soluble, as well as with herbal extracts that are not oil soluble. [0022] Examples of suitable herbal extracts that are oil soluble include, but are not limited to, chamomile, carrot root, echinacea purpurea, fennel, ginseng, grape seed, grape skin, grapefruit, guggalipids, harpogophytum, jasmine, mar- US 2003/0082116 A1 joram, myrrh gum resin, onion, pine bark, red clover flower, resveratrol, rosemary, sesame, St. Johns wort, thyme, Uva Ursi (bearberry), borage seed oil, wild borage seed oil, hesperedin, quercetin, kaempherol, genistein, coumestrol, estriol, phytosterols, limonene, ethoxyquin, hydroquinone, ubiquinone (coenzyme Q), lipoic acid, N-acetyl cysteine, curcumin, derivatives thereof, mixtures thereof, and the like. Examples of suitable herbal extracts that are oil soluble and water soluble include, but are not limited to, basil leaf, bell pepper, dandelion root, date palm fruit, licorice, tomato, myricetin, derivatives thereof, mixtures thereof, and the like. Examples of suitable herbal extracts that are water soluble include, but are not limited to, black tea extracts, blackberry, black currant fruit, coffee seed, gingko leaf, green tea polyphenyls (such as epicatechin gallate and epigallocaat- echin 3-O-gallate), hawthorn berries, sage, strawberry, sweet pea, vanilla fruit, neohesperidin, rutin, morin, chlo- rogenic acid, glutathione, derivatives thereof, mixtures thereof, and the like. [0023] Herbal extracts particularly effective for sebum/oil control include dill, horseradish, oats, neem, beet, broccoli, tea, pumpkin, soybean, barley, walnut, flax, ginseng, poppy, avocado, pea, sesame, dandelion, wheat, nettle, cashew, pineapple, apple, asparagus, Brazilnut, chickpea, grapefruit, orange, cucumber, buckwheat, strawberry, ginko, tomato, blueberry, cowpea and grape extracts. Other herbal extracts also suitable are those of ivy horse chestnut, centella asi- atica, rosmarinic acid, glycyrrizinate derivatives, alpha bis- abolol, azulene and derivatives thereof, asiaticoside, serico- side, ruscogenin, escin, escolin, betulinic acid and derivatives thereof, and catechin and derivatives thereof. [0024] In embodiments, a preferred dual function stabi- lizer is curcumin, or derivatives thereof such as curcumi- noids. Curcumin, also referred to as tumeric yellow, difeur- loylmethane, or C. I. Natural Yellow 3, provides the dual benefits of being a stabilizing agent to the adhesive com- position prior to its application, as well as providing anti- microbial effects during and after application. In addition, curcumin compounds provide a characteristic color to the composition, which can assist in identifying where the adhesive composition has been applied, to help ensure proper placement and coverage. Suitable curcumin and curcumin derivatives include, but are not limited to, cur- cumin, demethoxycurcumin, bisdemethoxycurcumin, tet- rahydrocurcumin, tetrahydrodemethoxycurcumin, tetrahy- drobisdemethoxycurcumin, other curcumin metabolites, (4-hydroxy-3-methoxycinnamoyl)methane, bis(4-hydroxy- cinnamoyl)methane, hexahydrocurcumin, octahydrocur- cumin, mixtures thereof, and the like. [0025] According to other embodiments of the present invention, the dual function stabilizer can be a selected alpha- or beta-hydroxycarboxylic acid, such as alpha- and beta-hydroxycarboxylic acids ranging from C2-C30. The beta-hydroxycarboxylic acids are primarily exemplified by salicylic acid and C1-C30 ester and salt derivatives. Examples of suitable alpha-hydroxycarboxylic acids include but are not limited to: alpha hydroxy acetic acid (glycolic acid), alpha hydroxybenzeneacetic acid (mandelic acid), alpha hydroxypropionic acid (lactic acid), alpha hydroxybu- tanoic acid, alpha hydroxyhexanoic acid, alpha hydroxyoc- tanoic acid (alpha hydroxycaprylic acid), alpha hydrox- ynonanoic acid, alpha hydroxydecanoic acid, alpha hydroxyundecanoic acid, alpha hydroxydodecanoic acid May 1, 2003 (alpha hydroxylauric acid), alpha hydroxytetradecanoic acid, alpha hydrocyhexadecanoic acid, alpha hydroxyocta- decanoic acid, alpha hydroxyoctaeicosanoic acid, dicar- boxylic alpha hydroxy acids, dihydroxybutanedioic acid (tartaric acid), 2-hydroxybutanedioic acid (malic acid), 2-hydroxy propanedioic acid, 2-hydroxy hexanedioic acid, 2-hydroxy octanedioic acid, 2-hydroxy decanedioic acid, 2-hydroxy dodecanedioic acid, 2-hydroxy myristicdioic acid, 2-hydroxy palmiticdioic acid, tricarboxylic alpha hydroxy acid, 2-hydroxy-1,2,3,-propanetricarboxylic acid (citric acid), 1-hydroxy-1,2,3-propanetricarboxylic acid (isocitric acid) and mixtures thereof. [0026] C1-C30 esters and salts of alpha- and beta-hydroxy- carboxylic acids (e.g. potassium, sodium, ammonium, tri- ethanolammonium and the like salts) are also meant to be included within the term “alpha- and beta-hydroxycarboxy- lic acid.” Depending on the pH of the composition, a mixture of the salt and the acid may be present. [0027] According to the present invention, preferred alpha hydroxycarboxylic acids are monocarboxylic acids, in order to improve skin penetration and efficacy. Even more pref- erably, the hydroxy acid is in embodiments chosen from lactic acid, glycolic acid, mandelic acid, and mixtures thereof to optimize the efficacy of compositions by increas- ing percutaneous absorption. Most preferred is the L-form of an alpha hydroxycarboxylic acid. [0028] Ceramides useful for the present invention are sphingolipids or phytosphingolipids including Ceramide 1, Ceramide 3and Ceramide 6. [0029] Anti-inflammatories of the present invention are illustrated by corticoids such as beta-methasone 17-acetate, indomethacin, ketoprofen, flufenamic acid, ibuprofen, diclofenace, diflunisal, fenclofenac, naproxen, piroxidam and sulindac. Vasoconstrictors are illustrated by compounds such as papaverine, yohimbine, visnadin, khellin, bebellin and nicotinate derivatives. Other substances within one or more of the above categories of actives include resorcinol, azelaic acid, oxamic acid and cyoctol. [0030] The amount of dual function stabilizer that is added to the monomer composition depends on the monomer to be stabilized, the stabilizer being selected, and/or the packaging material to be used for the composition. Preferably, the dual function stabilizers of the present invention may be included in an amount of from about 0.00001 to about 40%, prefer- ably from about 0.01 to about 20%, optimally from about 0.1 to about 10% and in some instances from about 1 to about 8% by weight of the composition. Of course, herbal extracts are usually employed at much lower levels than for instance the hydroxycarboxylic acids. Thus, the amount of herbal extracts may range from about 0.0001 to about 1%, prefer- ably from about 0.001 to about 0.5% by weight. Preferably the amount of the hydroxycarboxylic acid component, when present in the composition according to the invention, is from about 0.5% to about 20%, more preferably from about 1% to about 15%, and most preferably from about 3.0% to about 12.0% by weight of the composition. [0031] However, depending upon the desired stabilization and wound healing effects, lower amounts of the dual function stabilizer can be used. Thus, for example, the dual function stabilizer can be added in an amount of from about 0.01 to about 10 percent or more, preferably up to about 5 US 2003/0082116 A1 percent or more or 2.0 percent or more by weight based on the monomer. Contents outside of these ranges can be used, in embodiments, as desired. Thus, for example, where the antioxidants stabilizer is a higher molecular weight com- pound, greater amounts of the antioxidant can be used to obtain the desired result. [0032] Preferably, according to the present invention, the dual function stabilizer is included in the composition in an amount not only to provide effective stabilization of the monomeric adhesive composition, but also to provide wound healing properties to the composition. Thus, for example, the dual function stabilizer is included in an amount that provides sufficient residual antioxidant stabi- lizer in a formed polymeric material, such that the antioxi- dant can diffuse out of the formed polymer over time. By such diffusion, the stabilizer is made available to an adjacent tissue and/or a surrounding environment to impart wound healing properties. [0033] As used herein an amount of dual function stabi- lizer sufficient or effective “to stabilize” the monomer com- position refers to an amount of stabilizer sufficient to prevent the viscosity of a sterilized monomer composition from increasing to more than 200%, and preferably 150%, of the composition’s initial viscosity after sterilization. Suitable monomer composition stability, in terms of composition viscosity, according to the present invention is disclosed in U.S. patent application Ser. No. 09/374,207 filed Aug. 12, 1999, the entire disclosure of which is incorporated herein by reference. [0034] An indication of premature polymerization in 1,1- disubstituted ethylene monomer compositions, such as ot-cyanoacrylate monomer compositions in particular, is an increase in viscosity of the composition over time. That is, as the composition polymerizes, the viscosity of the com- position increases. If the viscosity becomes too high, i.e., too much premature polymerization has occurred, the compo- sition becomes unsuitable for its intended use or becomes very difficult to apply. Thus, while some polymerization or thickening of the composition may occur, such as can be measured by changes in viscosity of the composition, such change is not so extensive as to destroy or significantly impair the usefulness of the composition. However, the present invention, by providing a dual function stabilizer in the composition stored in the container, decreases or pre- vents the premature polymerization of the composition, and thereby provides better control over the viscosity of the composition. [0035] In embodiments of the present invention, the dual function stabilizer is included in the composition in an amount effective to provide effective stabilization of the composition. Thus, for example, the dual function stabilizer is contained in an amount effective to stabilize the compo- sition over a desired shelf-life of the product, and/or to stabilize the composition during and after any applicable sterilization procedure. A particular advantage of the stabi- lizers is that they provide effective stabilization of the composition during sterilization procedures, such as irradia- tion, dry heat, and/or chemical sterilization processes. Accordingly, and as necessary, the amount of the dual function stabilizer added to the composition can be varied depending upon, for example, the projected shelf-life of the composition and/or the kind and degree of selected steril- ization processing. Such selection and variation of the stabilizer can be performed by one of ordinary skill in the art with only routine experimentation. May 1, 2003 [0036] In embodiments of the present invention, particu- larly where the composition is to be used as a wound dressing or is otherwise being applied to tissue, the dual function stabilizer can be included in the composition for its wound healing properties as well as for its stabilization properties. In these embodiments, if desired, the amount of the stabilizer contained in the composition can be increased, so that an effective amount of the stabilizer remains present in the resultant polymer. When so present, the dual function stabilizer is generally released or diffuses from the polymer, either immediately or over time, into the tissue. Accordingly, one or more of the above-described additives can be included in the composition for their wound-healing effects. [0037] In addition, according to the present invention, the above-described dual function stabilizers can, additionally or alternatively, be included in a container that is used to contain polymerizable monomer adhesive compositions, such as 1,1-disubstituted adhesive compositions and cyanoacrylate adhesive compositions. The benefits of such inclusion, as well as a description of methods of including materials in the container itself, are disclosed in U.S. patent application Ser. No. 09/657,913, filed Sep. 8, 2000, the entire disclosure of which is incorporated herein by refer- ence. The methods disclosed therein for antioxidants and other stabilizers are also equally applicable to the dual function stabilizers of the present invention. Similar disclo- sure is also included in U.S. patent application Ser. No. 09/430,289, filed Oct. 29, 1999, the entire disclosure of which is incorporated herein by reference. [0038] According to the present invention, the container can be any suitable container used to contain a polymeriz- able monomeric adhesive composition. Thus, the container can be formed out of any suitable material, and in any shape, size and/or construction, as desired. Suitable container con- structions are disclosed, for example, in the above-refer- enced co-pending U.S. patent application Ser. Nos. 09/430, 289 and 09/657,913, the entire disclosure of which is incorporated herein by reference. [0039] In embodiments, the method of the present inven- tion further comprises sterilizing the 1,1-disubstituted eth- ylene monomer composition and/or its packaging, either prior to, or subsequent to, dispensing into the container. Suitable sterilization methods according to the present invention are disclosed in U.S. patent application Ser. No. 09/374,207 filed Aug. 12, 1999, the entire disclosure of which is incorporated herein by reference. [0040] Sterilization of the monomer composition and/or its packaging can be accomplished by techniques known to one of ordinary skill in the art, and is preferably accom- plished by methods including, but not limited to, chemical, physical, and/or irradiation methods. Examples of chemical methods include, but are not limited to, exposure to ethylene oxide or hydrogen peroxide vapor. Examples of physical methods include, but are not limited to, sterilization by heat (dry or moist) or retort canning. Examples of irradiation methods include, but are not limited to, gamma irradiation, electron beam irradiation, and microwave irradiation. A preferred method is electron beam irradiation, as described in U.S. Pat. No. 6,143,805, the entire disclosure of which is incorporated herein by reference, as well as gamma irradia- tion. The composition must show low levels of toxicity to living tissue during its useful life. In preferred embodiments of the present invention, the composition is sterilized to provide a Sterility Assurance Level (SAL) of at least 103. In embodiments, the Sterility Assurance Level may be at least 104, or may be at least 105, or may be at least 106. US 2003/0082116 A1 [0041] According to the invention, the combination of at least one dual function stabilizer provides sufficient inhibi- tion of polymerization of the monomer (i.e., stabilization of the composition) that sterility can be achieved without the unacceptable levels of polymerization or increases in cure rate due to over-stabilization that result from methods dis- closed in the prior art. For example, sterilized compositions according to embodiments of the present invention show an increase in viscosity of no more than 300%, and preferably less than 150%, as a result of sterilization. Viscosity levels can be determined by known techniques. For example, viscosity can be determined at room temperature (approxi- mately 21-25° C.) using a Brookfield Cone-Plate Viscom- eter. The instrument is standardized using a Viscosity Ref- erence Standard in the same range as the sample to be tested. Each sample is measured three times, and an average value determined and recorded. [0042] Preferred monomer compositions of the present invention, and polymers formed therefrom, are useful as tissue adhesives, sealants for preventing bleeding or for covering open wounds, and in other absorbable and non- absorbable biomedical applications. They find uses in, for example, apposing surgically incised or traumatically lacer- ated tissues; retarding blood flow from wounds; dressing burns; dressing skin or other superficial or surface wounds (such as abrasions, chaffed or raw skin, ulceration and/or stomatitis); hernia repair; meniscus repair; and aiding repair and re-growth of living tissue. Compositions of the present invention, and polymers formed therefrom, are also useful in industrial and home applications, for example in bonding rubbers, plastics, wood, composites, fabrics, and other natu- ral and synthetic materials. [0043] The monomer (including prepolymeric) adhesive composition may include one or more polymerizable mono- mers. Preferred monomers that may be used in this invention are readily polymerizable, e.g. anionically polymerizable or free radical polymerizable, or polymerizable by zwitterions or ion pairs to form polymers. Such monomers include those that form polymers, that may, but do not need to, biodegrade. Such monomers are disclosed in, for example, U.S. Pat. Nos. 5,328,687 and 5,928,611 to Leung et al., U.S. patent appli- cation Ser. No. 09/430,177, filed on Oct. 29, 1999, and U.S. Pat. No. 6,183,593, which are hereby incorporated in their entirety by reference herein. Preferred monomers include 1,1-disubstituted ethylene monomers, such as ot-cyanoacry- lates including, but not limited to, alkyl ot-cyanoacrylates having an alkyl chain length of from about 1 to about 20 carbon atoms or more, preferably from about 3 to about 8 carbon atoms. [0044] The ot-cyanoacrylates of the present invention can be prepared according to several methods known in the art. U.S. Pat. Nos. 2,721,858, 3,254,111, 3,995,641, and 4,364, 876, each of which is hereby incorporated in its entirety by reference herein, disclose methods for preparing ot-cy- anoacrylates. [0045] Preferred ot-cyanoacrylate monomers used in this invention include methyl cyanoacrylate, ethyl cyanoacry- late, n-butyl cyanoacrylate, 2-octyl cyanoacrylate, methoxy- ethyl cyanoacrylate, ethoxyethyl cyanoacrylate, dodecyl cyanoacrylate, 2-ethylhexyl cyanoacrylate, butyl cyanoacry- late, 3-methoxybutyl cyanoacrylate, 2-butoxyethyl cyanoacrylate, 2-isopropoxyethyl cyanoacrylate, 1-meth- oxy-2-propyl cyanoacrylate, hexyl cyanoacrylate, or dode- cylcyanoacrylate. May 1, 2003 [0046] Suitable cyanoacrylates for use in the present invention also include, but are not limited to, alkyl ester cyanoacrylate monomers such as those having the formula CN HZC O O R1 0 R2 O R3 [0047] wherein R1 and R2 are, independently H, a straight, branched or cyclic alkyl, or are combined together in a cyclic alkyl group, and R3 is a straight, branched or cyclic alkyl group. Preferably, R1is H or a C1, C2 or C3 alkyl group, such as methyl or ethyl; R2 is H or a C1, C2 or C3 alkyl group, such as methyl or ethyl; and R3 is a C1-C16 alkyl group, more preferably a C1-C10 alkyl group, such as methyl, ethyl, propyl, butyl, pentyl, hexyl, heptyl, octyl, nonyl or decyl, and even more preferably a C2, C3 or C4 alkyl group. Such alkyl ester cyanoacrylates and other suitable monomers are disclosed in, for example, U.S. patent application Ser. Nos. 09/630,437, filed Aug. 2, 2000, and 09/919,877, filed Aug. 2, 2001, the entire disclosures of which are incorporated herein by reference. [0048] Examples of preferred alkyl ester cyanoacrylates include, but are not limited to, butyl lactoyl cyanoacrylate (BLCA), butyl glycoloyl cyanoacrylate (BGCA), ethyl lac- toyl cyanoacrylate (ELCA), and ethyl glycoloyl cyanoacry- late (EGCA). BLCA may be represented by the above formula, wherein R1 is H, R2 is methyl and R3 is butyl. BGCA may be represented by the above formula, wherein R1 is H, R2 is H and R3 is butyl. ELCA may be represented by the above formula, wherein R1 is H, R2 is methyl and R3 is ethyl. EGCA may be represented by the above formula, wherein R1 is H, R2 is H and R3 is ethyl. [0049] The composition may optionally also include at least one other plasticizing agent that assists in imparting flexibility to the polymer formed from the monomer. The plasticizing agent preferably contains little or no moisture and should not significantly affect the stability or polymer- ization of the monomer. Examples of suitable plasticizers include but are not limited to isopropyl myristate, isopropyl palimtate, tributyl citrate, acetyl tri-n-butyl citrate (ATBC), polymethylmethacrylate, polydimethylsiloxane, polyester glutarates; polyester adipates; polyester sebacates; and oth- ers as listed in U.S. Pat. No. 6,183,593, the disclosure of which is incorporated in its entirety by reference herein. [0050] The composition may also optionally include at least one thixotropic agent. Suitable thixotropic agents are known to the skilled artisan and include, but are not limited to, silica gels such as those treated with a silyl isocyanate, and optionally surface treated titanium dioxide. Examples of suitable thixotropic agents and thickeners are disclosed in, for example, U.S. Pat. No. 4,720,513, and U.S. patent application Ser. No. 09/374,207 filed Aug. 12, 1999, the disclosures of which are hereby incorporated in their entire- ties by reference herein. US 2003/0082116 A1 [0051] The composition may optionally also include thickeners. Suitable thickeners may include poly (2-ethyl- hexy methacrylate), poly(2-ethylhexyl acrylate) and others as listed in U.S. patent application Ser. No. 09/472,392 filed Dec. 23, 1999, the disclosure of which is incorporated by reference herein in its entirety. [0052] The composition may also optionally include at least one natural or synthetic rubber to impart impact resistance. Suitable rubbers are known to the skilled artisan. Such rubbers include, but are not limited to, dienes, styrenes, acrylonitriles, and mixtures thereof. Examples of suitable rubbers are disclosed in, for example, U.S. Pat. Nos. 4,313, 865 and 4,560,723, the disclosures of which are hereby incorporated in their entireties by reference herein. [0053] The composition may optionally also include one or more additional stabilizers, such as both at least one anionic vapor phase stabilizer and at least one anionic liquid phase stabilizer. These stabilizing agents may further inhibit premature polymerization. Suitable stabilizers may include those listed in U.S. Pat. No. 6,183,593, the disclosure of which is incorporated by reference herein in its entirety. Suitable stabilizing agents also include any of the known anionic and free radical stabilizing agents. [0054] Where an one or more additional stabilizers is included in the compositions, a preferred additional stabi- lizer in embodiments is an antioxidant stabilizer, such as those disclosed in U.S. patent application Ser. No. 09/657, 913, filed Sep. 8, 2000, the disclosure of which is incorpo- rated by reference herein in its entirety. [0055] Suitable antioxidant stabilizer can be selected from among known antioxidants, including, but not limited to, vitamin E (CZQHSOOZ) (including alpha-tocopherol (CZQHSOOZ), beta-tocopherol (C28H48O2), gamma-toco- pherol (C28H48O2) and delta-tocopherol (C27H46O2) and derivatives thereof, vitamin K (including phylloquinone (C31H46O2), menaquinone (e.g. menaquinone 4 (C31H4O 02)), and menadione (C11H8O2) and derivatives thereof, including but not limited to vitamin K1, chromanol and vitamin K1 chromenol, vitamin C (ascorbic acid (C6H8O6)) and derivatives thereof, pentamethyl chromanol (C14H2OO2), non-phenolic antioxidants, octyl gallate (C14H20O5) and pentamethyl benzofuranol (C13H18O2). A preferred vitamin E antioxidant is any of the series of IRGANOX® brand vitamin E (available from Ciba Spe- cialty Chemical Co.). Apreferred pentamethyl chromanol is 2,2,5,7,8-pentamethyl-6-chromanol. Derivatives of the described compounds, particularly where the moieties are benzopyranols or benzofuranols, are also suitable. [0056] Other suitable antioxidant stabilizers include com- pounds of the following formula: R1 H0 [0057] where R1, R2, R3, R4, and R5 are independently selected from hydrogen, substituted alkyl or unsubstituted May 1, 2003 alkyl groups having from, for example, 1 to about 20 carbon atoms. Furthermore, suitable compounds include those of the above formula where R2 and R3, and/or R4 and R5, may optionally form cyclic groups having from about 2 to about 40 carbon atoms, preferably from about 2 or about 3 to about 6 or about 8 carbon atoms. [0058] The compositions may also include pH modifiers to control the rate of degradation of the resulting polymer, as disclosed in U.S. patent application Ser. No. 08/714,288, filed Sep. 18, 1996, the entire disclosure of which is hereby incorporated by reference herein in its entirety. [0059] Compositions of the present invention may also include at least one biocompatible agent effective to reduce active formaldehyde concentration levels produced during in vivo biodegradation of the polymer (also referred to herein as “formaldehyde concentration reducing agents”). Preferably, this component is a formaldehyde scavenger compound. Examples of formaldehyde scavenger com- pounds useful in this invention include sulfites; bisulfites; mixtures of sulfites and bisulfites, etc. Additional examples of formaldehyde scavenger compounds useful in this inven- tion and methods for their implementation can be found in U.S. Pat. Nos. 5,328,687, 5,514,371, 5,514,372, 5,575,997, 5,582,834 and 5,624,669, all to Leung et al., which are hereby incorporated herein by reference in their entireties. [0060] To improve the cohesive strength of adhesives formed from the compositions of this invention, difunctional monomeric cross-linking agents may be added to the mono- mer compositions of this invention. Such crosslinking agents are known. U.S. Pat. No. 3,940,362 to Overhults, which is hereby incorporated herein in its entirety by refer- ence, discloses exemplary cross-linking agents. [0061] The compositions of this invention may further contain a fibrous reinforcement and colorants such as dyes, pigments, and pigment dyes. Examples of suitable fibrous reinforcements include PGA microfibrils, collagen microfibrils, and others as described in U.S. Pat. No. 6,183, 593, the disclosure of which is incorporated by reference herein in its entirety. [0062] The polymerizable compositions useful in the present invention may also further contain one or more medicaments, preferably one or more non-antioxidant medi- caments. Suitable medicaments include, but are not limited to, antibiotics, antimicrobials, antiseptics, bacteriocins, bac- teriostats, disinfectants, steroids, anesthetics, antifungal agents, anti-inflammatory agents (other than the dual func- tion stabilizers of the present invention), antibacterial agents, antiviral agents, antitumor agents, growth promoting substances, antioxidants (other than the dual function stabi- lizers of the present invention), or mixtures thereof. Suitable specific medicaments are disclosed in, for example, U.S. patent application Ser. No. 09/430,177, filed Oct. 29, 1999, the entire disclosure of which is incorporated herein by reference. [0063] The polymerizable compositions useful in the present invention may also further contain one or more preservatives. The preservatives may be present, for example, for prolonging the storage life of the composition and/or for destroying and/or usefully suppressing the growth or metabolism of a variety of microscopic or submicroscopic life forms, either in the composition itself, or in or on a US 2003/0082116 A1 substrate to which the composition may be applied. Suitable preservatives, and methods for selecting them and incorpo- rating them into adhesive compositions, are disclosed in U.S. patent application Ser. No. 09/430,180, the entire disclosure of which is incorporated herein by reference. [0064] In embodiments of the present invention, the com- position and/or its applicator may contain materials such as a polymerization initiator, accelerator, rate-modifier, and/or cross-linking agent for initiating polymerization and/or cross-linking of the polymerizable monomer material. Suit- able materials and applicators and packaging systems are disclosed in U.S. Pat. No. 5,928,611 and U.S. patent appli- cation Ser. Nos. 09/430,177, 09/430,176, 09/430,289, 09/430,290, and 09/430,180 filed Oct. 29, 1999; 09/343,914 filed Jun. 30, 1999; 09/385,030 filed Aug. 30, 1999; and 09/176,889 filed Oct. 22, 1998; the entire disclosures of which are incorporated herein by reference. EXAMPLES Example 1 [0065] A 2-octyl Cyanoacrylate monomer (2-OCA) com- position is prepared by the addition of 0.5 wt. percent of curcumin. The monomer composition is stirred until all the curcumin is dissolved. The curcumin dissolves in the 2-OCA to provide a medically acceptable monomer composition. The formulation is suitable for evaluated of its wound healing property. Example 2 [0066] Pigs are anesthetized and the entire dorsal, thoracic region is shaved using an electric clipper. Portions of the area are shaved using a single edge razor and soap as a lubricant. Care is taken to avoid abrasions during shaving. The shaved surface is antiseptically prepared using a non- antimicrobial soap solution followed by 3 rinses with water and a final rinse with 70% isopropyl alcohol. Wounds measuring approximately 0.7 cm>
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